Early detection of Genotoxicity: Integrated Services from Gentronix.

Testing for genotoxicity is a regulatory requirement, historically conducted late in the preclinical phase of drug development. Such testing using the regulatory battery of tests is time consuming and costly with positive results leading to delays or even termination of development. Gentronix provides a comprehensive early genotoxicity screening using cell based assays and in vitro genotoxicity assays.

GreenScreen HC and BlueScreen HC 

Early accurate screening conducted with GreenScreen® HC or BlueScreen HC™ human (TK6) cell based assays gives rapid results. Both assays use patented gene reporter systems with either Green Fluorescent Protein (GFP) or Gaussia Luciferase (G.Luc) to detect up-regulation of GADD-45a, a key P53 dependant response element in the DNA stress pathway. The assays require only milligram quantities of material and give results in just 48hrs.

In vitro Micronucleus Test

The micronucleus assay detects aberrant, small nuclei present in the cell cytoplasm. Micronuclei may contain whole chromosomes ‘lost’ during mitosis or fragments generated by chromosome breakage.

Flow cytometric assessment permits automated scoring, which removes subjectivity, and rapid measurement of large numbers of cells increases statistical power. In addition, the in vitro MicroFlow® method used by Gentronix allows simultaneous collection of relative survival and membrane integrity parameters for cytotoxicity assessment, as well as cell cycle information. 


Ames MPF assay
  
Ames MPF™ is an adapted microfluctuation method for performing the Ames bacterial mutagenicity test. Hisˉ or Trpˉ tester strains are incubated with test samples and scored for colony growth, indicating reversion to His+ or Trp+ phenotype has occurred.

Project Day 1

Can we kill skin cancer cells (the bad guys) without harming normal cells (the good guys)?

Normally we remove cancer with surgery (a doctor cuts it out). This project explores the possibility of using drugs to remove cancer cells.

Drugs designed to affect cellular functions unique to cancer cells are called “targeted therapies”.

We previously compared the genes switched on in skin cancer cells with genes switched on in normal skin cells and identified 2 genes which are important for keeping cancer cells alive.

produces Polo-like kinase-1 which is an enzyme that plays a role in cell division (mitosis). This protein has a known role in maintaining cancer cell survival in cancers from organs other than the skin. As a consequence small molecule inhibitors which bind to the active site of the enzyme have been developed by drug companies. We will test whether skin cancer and normal skin cells are affected by exposing these cells to two inhibitors, BI25436 and GW843682X.

is a gene with unknown function. Eventually my lab wants to develop drugs which target the protein product of this gene but before this can happen we need to know what job it does in the cell. To do this we can introduce DNA which will make the cell produce C20ORF20 protein that has a fluorescent tag attached to it. This fluorescent tag will tell us where the protein goes giving us a clue to what it does in the cell. We will manipulate this DNA and introduce it into skin cells to look at where the protein goes in the cell using fluorescent microscopy.


Gfp Gel Electrophoresis - Bookshelf

Electrophoresis in practice, a guide to methods and applications of DNA and protein separations

Electrophoresis in practice, a guide to methods and applications of DNA and protein separations

Creator: Reiner Westermeier | Science -
This laboratory guide for successful electrophoretic separations is divided into two parts to provide readers with a thorough presentation of the fundamentals ...
Publisher: Vch Verlagsgesellschaft Mbh

Electrophoresis, theory, methods, and applications

Electrophoresis, theory, methods, and applications

Creator: Milan Bier | Science -

Electrophoresis, theory, techniques, and biochemical and clinical applications

Electrophoresis, theory, techniques, and biochemical and clinical applications

Creator: Anthony T. Andrews | Science -
Publisher: Oxford University Press, USA

Handbook of sol-gel science and technology, processing, characterization and applications

Handbook of sol-gel science and technology, processing, characterization and applications

Creator: Sumio Sakka | Science -
There is a growing need for a comprehensive reference that treats both the fundamentals and the applications, and this is the aim of "Handbook of Sol-Gel ...
Publisher: Springer

Gel electrophoresis of proteins, a practical approach

Gel electrophoresis of proteins, a practical approach

Creator: B. D. Hames | Fiction -
This new edition is almost a completely new text, with eight of the ten chapters written by new authors.
Publisher: Oxford University Press, USA

Daily Data Directory


GFP Purification
The altered phenotype, of course, tells us that the GFP gene is being expressed. ... the purity of our preparations by polyacrylamdine gel electrophoresis (PAGE) ...

Direct Immunodetection of Antigens within a Polyacrylamide Gel
Figure 2. In-gel immunodetection using biotinylated goat anti-GFP* antibody. ... gel and separated by electrophoresis. The gel was probed with GFP ...

Biotechnology Explorer
In the first part of the exercise, a plasmid encoding the GFP protein is ... technique of gel electrophoresis is used to separate the entire repertoire of proteins ...

PowerPoint Presentation
Your GFP with flow through the column, closer and closer towards the bottom of ... compare by agarose gel electrophoresis the new GFP plasmid to the old ...

Gel Electrophoresis
Gel Electrophoresis a. Theory b. Components Required i. Matrix ii. ... Epitope tagging and GFP fusion Multiple proteins: 2D- gel electrophoresis MALDI-TOF mass ...